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Article Released Wed-21st-September-2005 12:53 GMT
Contact: Dr Sharifah Syed Hassan Institution: Veterinary Research Institute
 New serological technique for large scale surveillance of bird flu virus

The I-ELISA is very suitable for large scale surveillance for avian influenza and can be used to detect antibody positive chickens if an outbreak of Avian Influenza Virus is suspected or for surveillance purposes post-outbreak at a particular location.

For more details, please contact:

Jamal Abdul Nasir Mohamad Hassan.
Email: JAMAL@iphvri.po.my

Title of research paper: Comparison between Indirect ELISA (I-ELISA), Competitive ELISA (C-ELISA), and Hemagglutination Inhibition Test (HI) for Detection of Antibodies to Avian Influenza Virus in Chicken Sera from Australian 1992, AIV H7N3 Outbreak.

Authors: Jamal Abdul Nasir Mohamad Hassan (1), Paul W. Selleck (2) and Sohayati Abdul Rahman (1)
(1) Veterinary Research Institute, 59, Jalan Sultan Azlan Shah, 31400 Ipoh Perak Malaysia
(2) CSIRO Australian Animal Health Laboratory, 5, Portarlington Road, Geelong, 3220, Victoria, Australia.


Avian influenza (AI) is a very devastating disease to the poultry industry causing high mortalities and morbidities in its peak and also have public health repercussions as shown in the Hong Kong flu outbreak in 1997, and the outbreaks through South East Asia (2003 till now), that had caused human lives and great economic impact.

Detection of the virus in infected flocks is very crucial in the surveillance program for disease control. Isolation of the agent is highly recommended as means of identifying infected farms. However, serological surveys are also recommended so that large number of samples can be screened in many farms and only the antibody positive farms are focused for further isolation work.

We compare between the Malaysian developed Indirect Enzyme Linked Immunosorbent Assay (iELISA) with the Australian CSIRO developed Competitive Enzyme Linked Immunosorbent Assay (cELISA) and the Haemagglutination Inhibition Test (HI) for detection of antibodies to type A influenza virus in chicken sera in Australian 1992 avian influenza virus outbreak which was caused by avian influenza virus serotype H7N3

Serum samples used in this work was supplied by the CSIRO Australian Animal Health Laboratory, Victoria Australia, which were collected from 60 chickens during the Australian 1992 avian influenza virus outbreak which was caused by avian influenza virus serotype H7N3.

This research found that iELISA detected more positives than the cELISA and the HI. This indicates that sensitivity of the iELISA is higher than the cELISA and HI for detection of antibodies in these samples.

Conclusion

The iELISA developed in the Veterinary Research Institute Ipoh has good correlation with the Australian Competitve Enzyme Linked Immunosorbent Assay (cELISA) and Hemagglutination Inhibition Test (HI) for detection of antibodies to type A influenza virus in chicken sera. The I-ELISA can detect all the positive sera that were detected positive by both the other test

The I-ELISA detected one extra positive serum that was negative in both the other test. Considering its sensitivity, the I-ELISA is very suitable for large scale surveillance for avian influenza and can be used to detect antibody positive chickens if an outbreak of Avian Influenza Virus is suspected or for surveillance purposes post-outbreak at a particular location.

Meeting information

Colloquium on Viruses of Veterinary & Public Health Importance; 11-12 January 2005

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