Medicine research news Return to previous page
Article Released Wed-23rd-November-2005 15:52 GMT
Contact: Dr Sharifah Syed Hassan Institution: Veterinary Research Institute
 Molecular identification of an Avian Influenza Virus (AIV) Subtype H6 Isolated from Cloacal Swabs of Ducks

A practical approach for the isolation and molecular identification of bird flu virus from duck cloaca swabs submitted during the surveillance of bird flu in Malaysia.

Authors: Suriani, M.N., Norfaizah, A.H.(*), Sharifah, S.H., Ong, G.H., Rafidah, A.J. and N.Asiah(*)

Veterinary Research Institute, 59, Jalan Sultan Azlan Shah, 31400, Ipoh, Perak.
Regional Veterinary Laboratory, Department of Veterinary Services, 46630, Petaling Jaya, Selangor.

Abstract
Highly pathogenic Avian influenza or bird flu is a rapidly spreading fatal disease in the avian species as well as in human. An early detection of the virus will improve the control of influenza in animal and human. Routinely bird flu virus infection is diagnosed by conventional method which requires several days to produce a conclusive result.

In this paper we report a practical approach for the isolation and molecular identification of bird flu virus from duck cloaca swabs submitted during the surveillance of bird flu in Malaysia. The isolation of the virus was conducted in embryonating SPF (specific pathogen free) chicken eggs which showed embryonic eggs death after the second passage.

Haemagglutination activities were observed without any inhibition activities using specific antisera for Newcastle disease, Adenovirus and subtype specific antisera for bird flu (H3, H5, H7 and H9).

Due to this limitation, real time polymerase chain reaction (RRT-PCR) for detection of bird flu infection which allow rapid differentiation between low-pathogenic avian influenza (LPAI) and HPAI viruses was established. RRT-PCR was performed using published universal primers of matrix gene and specific primer which were designed to amplify the HA genes of H5, H6, H9 and H7 subtypes.

Meeting information

11th International Conference of the Association of Institutions for Tropical Veterinary Medicine and 16th Veterinary Association Malaysia Congress, 23-27th August, Sunway Pyramid Convention Centre, Petaling Jaya, Malaysia

Keywords associated to this article:
Create Account...